Journal: The Journal of toxicological sciences
Article Title: Segment-specific and direction-dependent transport of cadmium and manganese in immortalized S1, S2, and S3 cells derived from mouse kidney proximal tubules.
doi: 10.2131/jts.44.611
Figure Lengend Snippet: Fig. 1. Western blot analyses of segment-specific marker pro- teins and metal transporters. Whole-cell lysates of S1, S2, and S3 cells were subjected to Western blot analy- ses. β-actin was used as a loading control. (A) The pro- tein levels of markers for each segment such as OCT1 for the S1 segment, CD98 for the S1 and S2 segments, and SNAT3 and GS for the S3 segment showed similar expression patterns as those observed in in vivo stud- ies. (B) The protein level of ZIP8 was the highest in S3 cells, while those of ZIP14 and DMT1 were rough- ly similar among S1, S2, and S3 cells.
Article Snippet: Rabbit anti-ZIP8 polyclonal antibody was purchased from Proteintech (Chicago, IL, USA).
Techniques: Western Blot, Marker, Control, Expressing, In Vivo